試題詳解

19.Which of the following regarding restriction enzyme cleavage patterns is/are CORRECT?
(A) EcoRI produces 5' protruding (sticky) ends.
(B) EcoRV produces blunt ends
(C)Whether sticky or blunt ends are produced depends on the position of the cleavage site relative to the center of the symmetry sequence
(D)All restriction enzymes produce only blunt ends
(E)Sticky ends facilitate ligation efficiency when they are complementary to each other