試題詳解

16. A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme EcoRI, which cuts the plasmid at a single site in the tetracycline-resistance gene. The plasmid DNA is then annealed with an EcoRI digest of human DNA, ligated, and used to transform E. coli cells. What antibiotic would you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid?
(A) Ampicillin.
(B) Tetracycline.
(C) Ampicillin and tetracycline
(D) Any antibiotic will do.
(E) The agar plate should not contain any antibiotic.